Article
A mutation that converts serine340 of the HsdSK polypeptide to phenylalanine and its effects on restriction and modification in Escherichia coli K-12.
Gene - 31 May 1990
Zinkevich V E, Weiserová M, Kryukov V M, Hubácek J
Abstract excerpt
A hybrid hsdS gene, encoding the HsdSts + d polypeptide, was constructed by joining the proximal region of the wild-type (wt) hsdS sequence with the distal region of the hsdSts + d sequence, at the hsdS BglII site. The hybrid hsdS-Sts + d gene exerts a trans-dominant effect on restriction and modification, which points to the location of the temperature-sensitive (ts) trans-dominant (+ d) mutation in the gene...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA Mutational Analysis
- DNA Restriction-Modification Enzymes
- Escherichia coli
- Molecular Sequence Data
- Phenotype
- Phenylalanine
- Recombinant Proteins