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Article

The effect of recA mutation on the expression of EcoKI and EcoR124I hsd genes cloned in a multicopy plasmid.

Folia microbiologica - 1 Jan 1998

Hubácek J, Holubová I, Weiserová M

Abstract excerpt

Type I restriction-modification (R-M) endonucleases are composed of three subunits--HsdR, required for restriction, and HsdM and HsdS which can produce a separate DNA methyltransferase. The HsdS subunit is required for DNA recognition. In this paper we describe the effect of cloned EcoKI and EcoR124I hsd genes on the resulting R-M phenotype. The variability in the expression of the wild type (wt) restriction...

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Identifiers

PMID: 9821288
DOI: 10.1007/BF02818573

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Related research

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The effect of recA mutation on the expression of EcoKI and EcoR124I hsd genes cloned in a multicopy plasmid.PMID 9821288Read ↗

Regulation of the activity of the type IC EcoR124I restriction enzyme.Semantic proximity Read ↗

Restriction endonucleases R.EcoKI and R.EcoR124I are probably located in different environments within the bacterial cell.Semantic proximity Read ↗

Isolation of a non-classical mutant of the DNA recognition subunit of the type I restriction endonuclease R.EcoR124I.Semantic proximity Read ↗

A novel mutant of the type I restriction-modification enzyme EcoR124I is altered at a key stage of the subunit assembly pathway.Semantic proximity Read ↗

Cloning, production and characterisation of wild type and mutant forms of the R.EcoK endonucleases.Semantic proximity Read ↗

Overproduction of the Hsd subunits leads to the loss of temperature-sensitive restriction and modification phenotype.Semantic proximity Read ↗

Characterization of an EcoR124I restriction-modification enzyme produced from a deleted form of the DNA-binding subunit, which results in a novel DNA specificity.Semantic proximity Read ↗

A deletion mutant of the type IC restriction endonuclease EcoR1241 expressing a novel DNA specificity.Semantic proximity Read ↗

A residue of motif III positions the helicase domains of motor subunit HsdR in restriction-modification enzyme EcoR124I.Semantic proximity Read ↗

Regulation of endonuclease activity by proteolysis prevents breakage of unmodified bacterial chromosomes by type I restriction enzymes.Semantic proximity Read ↗

The DNA recognition subunit of the type IB restriction-modification enzyme EcoAI tolerates circular permutions of its polypeptide chain.Semantic proximity Read ↗

Nomenclature relating to restriction of modified DNA in Escherichia coli.Semantic proximity Read ↗

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