Article
The effect of recA mutation on the expression of EcoKI and EcoR124I hsd genes cloned in a multicopy plasmid.
Folia microbiologica - 1 Jan 1998
Hubácek J, Holubová I, Weiserová M
Abstract excerpt
Type I restriction-modification (R-M) endonucleases are composed of three subunits--HsdR, required for restriction, and HsdM and HsdS which can produce a separate DNA methyltransferase. The HsdS subunit is required for DNA recognition. In this paper we describe the effect of cloned EcoKI and EcoR...
Topics
- Bacterial Proteins
- Cloning, Molecular
- DNA Restriction Enzymes
- DNA Restriction-Modification Enzymes
- Deoxyribonucleases, Type I Site-Specific
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Magnesium Sulfate
- Mutation
- Plasmids
- Rec A Recombinases
- Temperature