Overproduction of the Hsd subunits leads to the loss of temperature-sensitive restriction and modification phenotype.

doi:10.1007/BF02814061

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Article

Overproduction of the Hsd subunits leads to the loss of temperature-sensitive restriction and modification phenotype.

Folia microbiologica - 1 Jan 1994

Weiserová M, Janscák P, Zinkevich V, Hubácek J

Abstract excerpt

The genes hsdM and hsdS for M. EcoKI modification methyltransferase and the complete set of hsdR, hsdM and hsdS genes coding for R. EcoKI restriction endonuclease, both with and without a temperature-sensitive (ts) mutation in hsdS gene, were cloned in pBR322 plasmid and introduced into E. coli C...

Topics

DNA Restriction Enzymes
Escherichia coli
Genes, Bacterial
Mutation
Phenotype
Plasmids
Site-Specific DNA-Methyltransferase (Adenine-Specific)
Temperature
Transformation, Bacterial

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