Article
Overproduction of the Hsd subunits leads to the loss of temperature-sensitive restriction and modification phenotype.
Folia microbiologica - 1 Jan 1994
Weiserová M, Janscák P, Zinkevich V, Hubácek J
Abstract excerpt
The genes hsdM and hsdS for M. EcoKI modification methyltransferase and the complete set of hsdR, hsdM and hsdS genes coding for R. EcoKI restriction endonuclease, both with and without a temperature-sensitive (ts) mutation in hsdS gene, were cloned in pBR322 plasmid and introduced into E. coli C (a strain without a natural restriction-modification (R-M) system). The strains producing only the methyltransferase,...
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