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Article

Mutation in the specificity polypeptide of the type I restriction endonuclease R.EcoK that affects subunit assembly.

Journal of molecular biology - 5 Oct 1992

Zinkevich V, Heslop P, Glover S W, Weiserova M, Hubácek J, Firman K

Abstract excerpt

We describe the isolation and characterization of a temperature-sensitive mutation within the hsdS gene of the type I restriction and modification system EcoK. This mutation appears to affect the ability of the HsdR subunit to interact with the HsdS subunit when forming an active endonuclease. We discuss the possibility that this mutant, together with another mutation described previously, may define a...

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PMID: 1404378
DOI: 10.1016/0022-2836(92)90210-b

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Mutation in the specificity polypeptide of the type I restriction endonuclease R.EcoK that affects subunit assembly.PMID 1404378Read ↗

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The DNA recognition subunit of the type IB restriction-modification enzyme EcoAI tolerates circular permutions of its polypeptide chain.Semantic proximity Read ↗

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Regulation of endonuclease activity by proteolysis prevents breakage of unmodified bacterial chromosomes by type I restriction enzymes.Semantic proximity Read ↗

A mutation that converts serine340 of the HsdSK polypeptide to phenylalanine and its effects on restriction and modification in Escherichia coli K-12.Semantic proximity Read ↗

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