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Article

Mutation in the specificity polypeptide of the type I restriction endonuclease R.EcoK that affects subunit assembly.

Journal of molecular biology - 5 Oct 1992

Zinkevich V, Heslop P, Glover S W, Weiserova M, Hubácek J, Firman K

Abstract excerpt

We describe the isolation and characterization of a temperature-sensitive mutation within the hsdS gene of the type I restriction and modification system EcoK. This mutation appears to affect the ability of the HsdR subunit to interact with the HsdS subunit when forming an active endonuclease. We...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
Deoxyribonucleases, Type I Site-Specific
Escherichia coli
Genes, Bacterial
Macromolecular Substances
Molecular Sequence Data
Mutation
Plasmids
Restriction Mapping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Temperature

Source

Identifiers

PMID: 1404378
DOI: 10.1016/0022-2836(92)90210-b

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1404378-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Mutation in the specificity polypeptide of the type I restriction endonuclease R.EcoK that affects subunit assembly.PMID 1404378Read ↗

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A novel mutant of the type I restriction-modification enzyme EcoR124I is altered at a key stage of the subunit assembly pathway.Semantic proximity Read ↗

Characterization of an EcoR124I restriction-modification enzyme produced from a deleted form of the DNA-binding subunit, which results in a novel DNA specificity.Semantic proximity Read ↗

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Overproduction of the Hsd subunits leads to the loss of temperature-sensitive restriction and modification phenotype.Semantic proximity Read ↗

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Temperature-sensitive mutants of the EcoRI endonuclease.Semantic proximity Read ↗

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