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Article

Genomic hsd-Mu(lac) operon fusion mutants of Escherichia coli K-12.

Gene - 1 Mar 1991

Prakash A, Valinluck B, Ryu J

Abstract excerpt

Genomic (chromosomal)hsd-Mu(lac) operon fusions have been constructed in two strains of Escherichia coli K-12 for the three hsd genes, hsdRK, hsdMK and hsdSK, using MudX and lambda placMu53. Expression of hsdK mutants ranged from 16 to 74 units (u) (with a mean of 52 u) for fusions to promoter pres and ranged from 26-75 u (also with a mean of 52 u) for fusions to promoter pmod. The expression of the two hsdK...

Topics

Bacteriophage mu
Chromosomes, Bacterial
Cloning, Molecular
Escherichia coli
F Factor
Genes, Bacterial
Lac Operon
Mutagenesis
Phenotype
Promoter Regions, Genetic
Restriction Mapping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 1827085
DOI: 10.1016/0378-1119(91)90027-9

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1827085-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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