Article
Genomic hsd-Mu(lac) operon fusion mutants of Escherichia coli K-12.
Gene - 1 Mar 1991
Prakash A, Valinluck B, Ryu J
Abstract excerpt
Genomic (chromosomal)hsd-Mu(lac) operon fusions have been constructed in two strains of Escherichia coli K-12 for the three hsd genes, hsdRK, hsdMK and hsdSK, using MudX and lambda placMu53. Expression of hsdK mutants ranged from 16 to 74 units (u) (with a mean of 52 u) for fusions to promoter pres and ranged from 26-75 u (also with a mean of 52 u) for fusions to promoter pmod. The expression of the two hsdK...
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