Cloning, production and characterisation of wild type and mutant forms of the R.EcoK endonucleases.

doi:10.1093/nar/21.3.373

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Article

Cloning, production and characterisation of wild type and mutant forms of the R.EcoK endonucleases.

Nucleic acids research - 11 Feb 1993

Weiserova M, Janscak P, Benada O, Hubácek J, Zinkevich V E, Glover S W, Firman K

Abstract excerpt

The hsdR, hsdM and hsdS genes coding for R.EcoK restriction endonuclease, both with and without a temperature sensitive mutation (ts-1) in the hsdS gene, were cloned in pBR322 plasmid and introduced into E.coli C3-6. The presence of the hsdSts-1 mutation has no effect on the R-M phenotype of this...

Topics

Cloning, Molecular
Deoxyribonucleases, Type I Site-Specific
Escherichia coli
Kinetics
Mutation
Operon
Plasmids
Site-Specific DNA-Methyltransferase (Adenine-Specific)
Temperature

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