Article
Control of the Escherichia coli rrnB P1 promoter strength by ppGpp.
The Journal of biological chemistry - 12 May 1995
Zhang X, Bremer H
Abstract excerpt
Fusions of the rrnB P1 and P2 promoters, and of the tandem P1-P2 combination, to a wild-type lacZ gene were constructed on plasmids and recombined into the mal region of the bacterial chromosome, close to the normal location and in the normal orientation of rrnB. The upstream activator region (Fis-binding sites) was always present with the P1 promoter, and all constructs contained the box A antitermination site...
Topics
- Chromosomes, Bacterial
- DNA-Directed RNA Polymerases
- Escherichia coli
- Genes, Bacterial
- Genotype
- Guanosine Tetraphosphate
- Kinetics
- Plasmids
- Promoter Regions, Genetic
- RNA, Bacterial
- Recombinant Fusion Proteins