Article
Characterization of RNA and DNA synthesis in Escherichia coli strains devoid of ppGpp.
The Journal of biological chemistry - 25 May 1993
Hernandez V J, Bremer H
Abstract excerpt
The synthesis rates of DNA, rRNA, bulk mRNA, protein, and RNA polymerase beta- and beta'-subunits were determined as functions of growth rate in a wild-type Escherichia coli strain, which produces guanosine tetraphosphate (ppGpp), and in a delta relA delta spoT mutant which does not produce ppGpp. The rate of stable RNA synthesis per amount of protein depends on three factors: RNA polymerase concentration, RNA...
Topics
- Chromosomes, Bacterial
- DNA, Bacterial
- DNA-Directed RNA Polymerases
- Escherichia coli
- Gene Deletion
- Genes, Bacterial
- Genotype
- Guanosine Tetraphosphate
- Kinetics
- Ligases
- Mathematics