Article
High-fold expansion of human cytotoxic T-lymphocytes specific for autologous melanoma cells for use in immunotherapy.
Journal of immunotherapy : official journal of the Society for Biological Therapy - 1 Dec 1991
Jotereau F, Pandolfino M C, Boudart D, Diez E, Dreno B, Douillard J Y, Muller J Y, LeMevel B
Abstract excerpt
We set up a culture protocol that consistently allows high-fold expansion of tumor-specific T-lymphocytes from most melanoma-invaded biopsies with low doses of recombinant interleukin-2 (rIL-2). Between 2-60 x 10(6) T-lymphocytes could be obtained and cryopreserved from 12 out of 13 patients, by culturing only 50 mm3 tumor tissue with rIL-2. Thawed lymphocytes from 11 of these patients could then be expanded by a...
Topics
- Cell Division
- Cytotoxicity, Immunologic
- Humans
- Immunotherapy, Adoptive
- Lymphocytes, Tumor-Infiltrating
- Melanoma
- Phenotype
- T-Lymphocytes, Cytotoxic
- Tumor Cells, Cultured