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Measurement of beta-galactosyltransferase activity in cell extracts with an ELISA-based assay.

Archives of biochemistry and biophysics - 1 Jul 1990

Stults C L, Macher B A

Abstract excerpt

An enzyme-linked immunosorbent assay (ELISA)-based glycosyltransferase assay has been used to measure UDP-Gal:N-acetylglucosamine beta-1,4-galactosyl-transferase (EC 2.4.1.38) activity in detergent extracts of chinese hamster ovary (CHO) cells. LEC11 cells (a mutant of the CHO cell line, Pro -5), which are known to express a complex array of carbohydrate structures, were used to develop the assay for use with...

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Identifiers

PMID: 2112899
DOI: 10.1016/0003-9861(90)90512-w

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Measurement of beta-galactosyltransferase activity in cell extracts with an ELISA-based assay.PMID 2112899Read ↗

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