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Article

Measurement of beta-galactosyltransferase activity in cell extracts with an ELISA-based assay.

Archives of biochemistry and biophysics - 1 Jul 1990

Stults C L, Macher B A

Abstract excerpt

An enzyme-linked immunosorbent assay (ELISA)-based glycosyltransferase assay has been used to measure UDP-Gal:N-acetylglucosamine beta-1,4-galactosyl-transferase (EC 2.4.1.38) activity in detergent extracts of chinese hamster ovary (CHO) cells. LEC11 cells (a mutant of the CHO cell line, Pro -5), which are known to express a complex array of carbohydrate structures, were used to develop the assay for use with...

Topics

Animals
Carbohydrate Sequence
Cell Line
Enzyme-Linked Immunosorbent Assay
Gangliosides
Glycosphingolipids
Humans
Kinetics
Lactose Synthase
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Microsomes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

N-Acetyllactosamine Synthase

Source

Identifiers

PMID: 2112899
DOI: 10.1016/0003-9861(90)90512-w

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2112899-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Measurement of beta-galactosyltransferase activity in cell extracts with an ELISA-based assay.PMID 2112899Read ↗

Chinese hamster ovary (CHO) cells may express six beta 4-galactosyltransferases (beta 4GalTs). Consequences of the loss of functional beta 4GalT-1, beta 4GalT-6, or both in CHO glycosylation mutants.Semantic proximity Read ↗

A mutation causing a reduced level of expression of six beta4-galactosyltransferase genes is the basis of the Lec19 CHO glycosylation mutant.Semantic proximity Read ↗

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