Article
Application of lectin microarray to crude samples: differential glycan profiling of lec mutants.
Journal of biochemistry - 1 Mar 2006
Ebe Youji, Kuno Atsushi, Uchiyama Noboru, Koseki-Kuno Shiori, Yamada Masao, Sato Takashi, Narimatsu Hisashi, Hirabayashi Jun
Abstract excerpt
We recently developed a novel system for lectin microarray based on the evanescent-field fluorescence-detection principle, by which even weak lectin-oligosaccharide interactions are detectable without a washing procedure. For its practical application, cell glycan analysis was performed for Chinese hamster ovary (CHO) cells and their glycan profile was compared with those of their glycosylation-defective Lec...
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