Article
In vivo degradation of a transcriptional regulator: the yeast alpha 2 repressor.
Cell - 18 May 1990
Hochstrasser M, Varshavsky A
Abstract excerpt
Metabolic instability is characteristic of regulatory proteins whose in vivo concentrations must vary as a function of time. The cell type-specific alpha 2 repressor of the yeast S. cerevisiae is shown here to have a half-life of only approximately 5 min. Each of the two structural domains of alpha 2 carries a sequence that can independently target a normally long-lived protein for rapid destruction. Moreover,...
Topics
- Amino Acid Sequence
- Fungal Proteins
- Gene Expression Regulation, Fungal
- Half-Life
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Protein Engineering
- Protein Processing, Post-Translational
- Recombinant Fusion Proteins
- Repressor Proteins