Site-directed mutagenesis by complementary-strand synthesis using a closing oligonucleotide and double-stranded DNA templates.

doi:10.1016/0003-2697(90)90279-i

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Article

Site-directed mutagenesis by complementary-strand synthesis using a closing oligonucleotide and double-stranded DNA templates.

Analytical biochemistry - 15 Feb 1990

Slilaty S N, Fung M, Shen S H, Lebel S

Abstract excerpt

An approach for generating structures capable of directing full-length complementary-strand synthesis for double-stranded plasmid DNA is described. The structures are formed following heat denaturation and cooling of linearized plasmid DNA molecules in the presence of what is referred to as a "cl...

Topics

Base Sequence
DNA
DNA, Bacterial
DNA-Directed DNA Polymerase
Escherichia coli
Molecular Sequence Data
Mutation
Oligonucleotides
Plasmids
Restriction Mapping
Templates, Genetic
Transformation, Bacterial
beta-Galactosidase

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