Article
Impact of mutation type and amplicon characteristics on genetic diversity measures generated using a high-resolution melting diversity assay.
The Journal of molecular diagnostics : JMD - 1 Jan 2013
Cousins Matthew M, Donnell Deborah, Eshleman Susan H
Abstract excerpt
We adapted high-resolution melting (HRM) technology to measure genetic diversity without sequencing. Diversity is measured as a single numeric HRM score. Herein, we determined the impact of mutation types and amplicon characteristics on HRM diversity scores. Plasmids were generated with single-base changes, insertions, and deletions. Different primer sets were used to vary the position of mutations within...
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