Article
Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus.
Journal of neuroimmunology - 1 May 1990
Blasi E, Barluzzi R, Bocchini V, Mazzolla R, Bistoni F
Abstract excerpt
A murine cell line (BV-2) has been generated by infecting primary microglial cell cultures with a v-raf/v-myc oncogene carrying retrovirus (J2). BV-2 cells expressed nonspecific esterase activity, phagocytic ability and lacked peroxidase activity. Such cells secreted lysozyme and, following appropriate stimulation, also interleukin 1 and tumor necrosis factor. Furthermore, BV-2 cells exhibited spontaneous...
Topics
- Animals
- Cell Transformation, Viral
- Cells, Cultured
- Interferon-gamma
- Macrophages
- Mice
- Mice, Inbred C57BL
- Neuroglia
- Oncogenes
- Phagocytosis
- Phenotype
- Retroviridae