Article
In vivo immortalization of murine peritoneal macrophages: a new rapid and efficient method for obtaining macrophage cell lines.
Journal of leukocyte biology - 1 Apr 1993
Adami C, Brunda M J, Palleroni A V
Abstract excerpt
Although several murine macrophage (m phi) cell lines from different sites have previously been obtained by in vitro infection with the J2 murine retrovirus, which carries the v-raf and v-myc oncogenes, it was not possible to immortalize thioglycolate-elicited peritoneal macrophages (Pm phi s) by...
Topics
- Animals
- Antigens, Surface
- Biomarkers
- Cell Line
- Cell Line, Transformed
- Clone Cells
- Culture Techniques
- Flow Cytometry
- Histocompatibility Antigens Class II
- Interferon-gamma
- Macrophages
- Mice
- Phenotype
- Recombinant Proteins
- Staining and Labeling