Immortalization and characterization of rat microglial cells.

doi:10.1111/j.1365-2990.1995.tb01064.x

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Article

Immortalization and characterization of rat microglial cells.

Neuropathology and applied neurobiology - 1 Aug 1995

McLaurin J, Almazan G, Williams K, Antel J P

Abstract excerpt

Microglial cell lines from rat brain were established by transfer of a temperature sensitive simian virus 40 large tumour antigen by means of a retrovirus. Four weeks after infection, colonies were generated in the presence of neomycin and granulocyte-macrophage colony stimulating factor (GM-CSF)...

Topics

Animals
Animals, Newborn
Antigens
Antigens, Polyomavirus Transforming
Brain
Cell Line
Cells, Cultured
Clone Cells
Immunohistochemistry
L-Lactate Dehydrogenase
Microglia
Phenotype
Rats
Tumor Necrosis Factor-alpha

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