Article
Hormone-dependent phosphorylation of the glucocorticoid receptor occurs mainly in the amino-terminal transactivation domain.
The Journal of biological chemistry - 5 Apr 1990
Hoeck W, Groner B
Abstract excerpt
Phosphorylation of glucocorticoid receptors is increased by hormone binding and has been implicated in transcriptional regulation. We performed a phosphoamino acid analysis and identified the phosphorylated regions of the glucocorticoid receptor with respect to its functional domains before and after hormone activation. Receptor was isolated by immunoprecipitation from [32P]orthophosphate-labeled FTO 2B rat...
Topics
- Animals
- Binding Sites
- Chymotrypsin
- Cyanogen Bromide
- Gene Expression
- Glucocorticoids
- Haplorhini
- Hydroxylamine
- Hydroxylamines
- Immunosorbent Techniques
- Liver Neoplasms, Experimental