Article
Fidelity variants of RNA dependent RNA polymerases uncover an indirect, mutagenic activity of amiloride compounds.
PLoS pathogens - 28 Oct 2010
Levi Laura I, Gnädig Nina F, Beaucourt Stéphanie, McPherson Malia J, Baron Bruno, Arnold Jamie J, Vignuzzi Marco
Abstract excerpt
In a screen for RNA mutagen resistance, we isolated a high fidelity RNA dependent RNA polymerase (RdRp) variant of Coxsackie virus B3 (CVB3). Curiously, this variant A372V is also resistant to amiloride. We hypothesize that amiloride has a previously undescribed mutagenic activity. Indeed, amiloride compounds increase the mutation frequencies of CVB3 and poliovirus and high fidelity variants of both viruses are...
Topics
- Amiloride
- Animals
- Antiviral Agents
- Base Sequence
- Chlorocebus aethiops
- DNA-Directed RNA Polymerases
- Enterovirus
- Genetic Variation
- HeLa Cells
- Humans
- Mutagenesis
- Mutagens
- RNA
- RNA, Viral