Article
The role of palmitoylation of the guanine nucleotide binding protein G11 alpha in defining interaction with the plasma membrane.
The Biochemical journal - 15 Sept 1995
McCallum J F, Wise A, Grassie M A, Magee A I, Guzzi F, Parenti M, Milligan G
Abstract excerpt
Mutations of Cys-9 to serine, Cys-10 to serine and a combination of both alterations were produced in a cDNA encoding murine G11 alpha to potentially interfere with the ability of the expressed polypeptides to act as substrates for post-translational palmitoylation. Each of these mutants and the wild-type protein were expressed in simian COS-1 cells. Mutation of either cysteine-9 or cysteine-10 decreased the...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Cell Membrane
- Chlorocebus aethiops
- Chromatography, Gel
- Cysteine
- Cytosol
- DNA Primers
- DNA, Complementary