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Article

Additive effect of single amino acid replacements on the kinetic stability of β-glucosidase B.

The protein journal - 1 Oct 2012

Camarillo-Cadena Menandro, Garza-Ramos Gerogina, Peimbert Mariana, Polaina Julio, Pérez-Hernández Gerardo, Zubillaga Rafael A

Abstract excerpt

Previously, we applied in vitro evolution to generate the thermoresistant triple mutant H62R/N223Y/M319I of β-glucosidase B (BglB) from Paenibacillus polymyxa. In order to dissect the energetic contributions to protein stabilization achieved by these mutations, we measured the kinetic constants of the heat denaturation of wild type BglB, the triple mutant and the three single mutants (H62R, N223Y, M319I) by...

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PMID: 22923194
DOI: 10.1007/s10930-012-9445-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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