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Article

Evaluation of protein adsorption and preferred binding regions in multimodal chromatography using NMR.

Proceedings of the National Academy of Sciences of the United States of America - 28 Sept 2010

Chung Wai Keen, Freed Alexander S, Holstein Melissa A, McCallum Scott A, Cramer Steven M

Abstract excerpt

NMR titration experiments with labeled human ubiquitin were employed in concert with chromatographic data obtained with a library of ubiquitin mutants to study the nature of protein adsorption in multimodal (MM) chromatography. The elution order of the mutants on the MM resin was significantly different from that obtained by ion-exchange chromatography. Further, the chromatographic results with the protein...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 20837551
DOI: 10.1073/pnas.1002347107
PMCID: PMC2947875

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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