Article
Strong and heterogeneous adsorption of infectious bursal disease VP2 subviral particle with immobilized metal ions dependent on two surface histidine residues.
Analytical chemistry - 15 Oct 2007
Doong Shyue-Ru, Chen Yi-Huei, Lai Su-Yuan, Lee Cheng-Chung, Lin Yu-Chiang, Wang Min-Ying
Abstract excerpt
VP2, the single outer protein of infectious bursal disease virus capsid, can self-assemble into T = 1 subviral particle (SVP), which can be efficiently purified by immobilized metal ion affinity chromatography (IMAC). In this study, a systemic investigation of the adsorption behavior of VP2 SVP on Ni-NTA resin was performed to identify that His253 and His249 on the surface of SVP are the key factors accounted for...
Topics
- Animals
- Cations
- Chromatography, Gel
- Diffusion
- Histidine
- Infectious bursal disease virus
- Microscopy, Electron
- Models, Molecular
- Mutation
- Nickel
- Porosity
- Protein Structure, Tertiary
- Serum Albumin, Bovine
- Surface Properties