Article
In vitro propagation and dynamics of T cells from skin biopsies by methods using interleukins-2 and -4 or anti-CD3/CD28 antibody-coated microbeads.
Acta dermato-venereologica - 1 Sept 2010
Hashizume Hideo, Hansen Anker, Poulsen Lars K, Thomsen Allan Randrup, Takigawa Masahiro, Thestrup-Pedersen Kristian
Abstract excerpt
In order to explore the mechanisms of inflammatory skin disorders, we established two methods of expanding skin-derived lymphocytes, one using high levels of interleukin (IL)-2 and IL-4 (method A) and the other using low levels of cytokines and anti-CD3/CD28 microbeads (method B). Both methods provide advantages for functional studies. With either of these two, we could obtain more than 10(7) cells/ from a 3 mm...
Topics
- Adult
- Aged
- Antibodies
- Biopsy
- CD28 Antigens
- CD3 Complex
- Cell Culture Techniques
- Cell Line
- Cell Proliferation
- Cell Separation
- Cytotoxicity, Immunologic
- Denmark
- Female
- Flow Cytometry