Article
Flow cytometric analysis of cytokine expression in short-term allergen-stimulated T cells mirrors the phenotype of proliferating T cells in long-term cultures.
Journal of immunological methods - 31 Aug 2011
Van Hemelen D, Oude Elberink J N G, Bohle B, Heimweg J, Nawijn M C, van Oosterhout A J M
Abstract excerpt
BACKGROUND: Allergen-specific T(H) cells play an important role in IgE-mediated disorders as allergies. Since this T(H) cell-population only accounts for a small percentage of T(H) cells, they are difficult to phenotype without prior selection or expansion. METHODS: Grass-pollen-specific T(H) cell profiles were evaluated in 5 allergic and 4 non-allergic individuals using three different approaches: CD154...
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