Article
PAR-CLIP and streamlined small RNA cDNA library preparation protocol for the identification of RNA binding protein target sites.
Methods (San Diego, Calif.) - 15 Apr 2017
Benhalevy Daniel, McFarland Hannah L, Sarshad Aishe A, Hafner Markus
Abstract excerpt
The study of protein-RNA interactions is critical for our understanding of cellular processes and regulatory circuits controlled by RNA binding proteins (RBPs). Recent next generation sequencing-based approaches significantly promoted our understanding of RNA biology and its importance for cell function. We present a streamlined protocol for Photoactivatable-Ribonucleoside-Enhanced Crosslinking and...
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