Article
The MIG1 repressor from Kluyveromyces lactis: cloning, sequencing and functional analysis in Saccharomyces cerevisiae.
FEBS letters - 4 Sept 1995
Cassart J P, Georis I, Ostling J, Ronne H, Vandenhaute J
Abstract excerpt
Sequence comparisons between Saccharomyces cerevisiae ScMig1 and Aspergillus nidulans CREA proteins allowed us to design two sets of degenerate primers from the conserved zinc finger loops. PCR amplification on Kluyveromyces marxianus and K. lactis genomic DNA yielded single products with sequenc...
Topics
- Amino Acid Sequence
- Aspergillus nidulans
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- Conserved Sequence
- DNA-Binding Proteins
- Kluyveromyces
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Repressor Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Sequence Analysis
- Zinc Fingers