Article
Construction and characterization of a Lactococcus lactis strain deficient in intracellular ClpP and extracellular HtrA proteases.
Microbiology (Reading, England) - 1 Sept 2006
Cortes-Perez N G, Poquet I, Oliveira M, Gratadoux J J, Madsen S M, Miyoshi A, Corthier G, Azevedo V, Langella P, Bermúdez-Humarán L G
Abstract excerpt
A Lactococcus lactis strain deficient in both its major proteases, intracellular (ClpP) and extracellular (HtrA), was constructed and characterized. This strain, hereafter called clpP-htrA, could be obtained only by conjugation between a clpP donor strain and an htrA recipient strain in the NZ9000 context, allowing heterologous gene expression under the control of the NICE (nisin-controlled expression) system....
Topics
- Conjugation, Genetic
- Endopeptidases
- Gene Expression Regulation, Bacterial
- Lactococcus lactis
- Models, Genetic
- Mutation
- Nisin
- Peptide Hydrolases