Article
Effective pragmatic approach of diagnosis of multidrug-resistant tuberculosis by high-resolution melt curve assay.
International journal of mycobacteriology - 1 Jan 2000
Negi Sanjay Singh, Singh Priyanka, Bhargava Anudita, Chandrakar Sachin, Gaikwad Ujjwala, Das Padma, Behra Ajoy
Abstract excerpt
Background: Effective management of multidrug-resistant tuberculosis (MDR-TB) requires cost-effective and rapid screening of rifampicin (RIF) and isoniazid (INH) resistance. Accordingly, a highly promising high-resolution melting (HRM) analysis was evaluated in the detection of mutation in rpoB, katG gene and inhA promoter region in Mycobacterium tuberculosis isolates. Methods: A total of 143 M. tuberculosis...
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