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Article

Rapid detection of rifampicin, isoniazid and streptomycin resistance in Mycobacterium tuberculosis clinical isolates by high-resolution melting curve analysis.

Journal of applied microbiology - 1 Oct 2012

Yadav R, Sethi S, Mewara A, Dhatwalia S K, Gupta D, Sharma M

Abstract excerpt

AIMS: This study was carried out to evaluate high-resolution melting (HRM) curve analysis assay for detection of mutations in three drug resistance-associated genes of Mycobacterium tuberculosis. METHODS AND RESULTS: Clinical isolates of Myco. tuberculosis phenotypically resistant to rifampicin (n = 29), isoniazid (n = 35) and streptomycin (n = 34) were analysed for mutations in rpoB, katG and rpsL genes,...

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PMID: 22747769
DOI: 10.1111/j.1365-2672.2012.05379.x

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