Article
Peptide affinity chromatography media that bind N(pro) fusion proteins under chaotropic conditions.
Journal of chromatography. A - 1 Oct 2010
Hahn Rainer, Seifert Michael, Greinstetter Sabine, Kanatschnig Barbara, Berger Eva, Kaar Waltraud, Jungbauer Alois
Abstract excerpt
To design a generic purification platform and to combine the advantages of fusion protein technology and matrix-assisted refolding, a peptide affinity medium was developed that binds inclusion body-derived N(pro) fusion proteins under chaotropic conditions. Proteins were expressed in Escherichia...
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