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Article

Peptide affinity chromatography media that bind N(pro) fusion proteins under chaotropic conditions.

Journal of chromatography. A - 1 Oct 2010

Hahn Rainer, Seifert Michael, Greinstetter Sabine, Kanatschnig Barbara, Berger Eva, Kaar Waltraud, Jungbauer Alois

Abstract excerpt

To design a generic purification platform and to combine the advantages of fusion protein technology and matrix-assisted refolding, a peptide affinity medium was developed that binds inclusion body-derived N(pro) fusion proteins under chaotropic conditions. Proteins were expressed in Escherichia coli using an expression system comprising the autoprotease N(pro) from Pestivirus, or its engineered mutant called...

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PMID: 20800233
DOI: 10.1016/j.chroma.2010.07.074

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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