Article

Extracellular production, purification and tag-removal of the N-terminally annexin- and ELP-tagged fluorescent proteins

2024-02-29

Abstract excerpt

<h4>Background: </h4> Rapid and cost-effective purification of the target protein for extracellular production in Escherichia coli is still challenge. Previously, we identified that human annexin A1 as a N-terminal fusion tag for Ca 2+ -dependent phase transition to simply, rapidly and cost-effectively purify three fluorescent proteins including emerald green fluorescent protein (EmGFP), red fluorescent protein mC...

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Literature Corpus work: b0690d06-5c8d-5e75-ba3e-2ffcc3ee5a62
DOI: 10.21203/rs.3.rs-3981174/v1

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