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Article

Peptides in headlock--a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy.

Scientific reports - 21 Jan 2016

Braun Michael B, Traenkle Bjoern, Koch Philipp A, Emele Felix, Weiss Frederik, Poetz Oliver, Stehle Thilo, Rothbauer Ulrich

Abstract excerpt

Nanobodies are highly valuable tools for numerous bioanalytical and biotechnical applications. Here, we report the characterization of a nanobody that binds a short peptide epitope with extraordinary affinity. Structural analysis reveals an unusual binding mode where the extended peptide becomes part of a β-sheet structure in the nanobody. This interaction relies on sequence-independent backbone interactions...

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Source

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Identifiers

PMID: 26791954
DOI: 10.1038/srep19211
PMCID: PMC4726124

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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