Article
Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids.
Nature communications - 23 Mar 2018
Martin Rey W, Des Soye Benjamin J, Kwon Yong-Chan, Kay Jennifer, Davis Roderick G, Thomas Paul M, Majewska Natalia I, Chen Cindy X, Marcum Ryan D, Weiss Mary Grace, Stoddart Ashleigh E, Amiram Miriam, Ranji Charna Arnaz K, Patel Jaymin R, Isaacs Farren J, Kelleher Neil L, Hong Seok Hoon, Jewett Michael C
Abstract excerpt
Cell-free protein synthesis has emerged as a powerful approach for expanding the range of genetically encoded chemistry into proteins. Unfortunately, efforts to site-specifically incorporate multiple non-canonical amino acids into proteins using crude extract-based cell-free systems have been limited by release factor 1 competition. Here we address this limitation by establishing a bacterial cell-free protein...
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