Hybridisation thermodynamic parameters allow accurate detection of point mutations with DNA microarrays.

Abstract excerpt

We consider mixtures of two DNA sequences t and t' differing by a single nucleotide, which are analyzed by an Agilent custom DNA microarray. In particular we focus on the case in which t, the "wild type", is predominantly abundant and t' the "mutant" is at very low concentrations compared to t. We show that by using appropriately designed arrays it is possible to accurately quantify the presence of t' even at low...

Topics

• Base Pair Mismatch
• Base Sequence
• DNA Probes
• Nucleic Acid Hybridization
• Oligonucleotide Array Sequence Analysis
• Point Mutation
• Polymerase Chain Reaction
• Polymorphism, Single Nucleotide
• Thermodynamics