Article
Coelenterazine-v ligated to Ca2+-triggered coelenterazine-binding protein is a stable and efficient substrate of the red-shifted mutant of Renilla muelleri luciferase.
Analytical and bioanalytical chemistry - 1 Oct 2010
Stepanyuk Galina A, Unch James, Malikova Natalia P, Markova Svetlana V, Lee John, Vysotski Eugene S
Abstract excerpt
It has been shown that the coelenterazine analog, coelenterazine-v, is an efficient substrate for a reaction catalyzed by Renilla luciferase. The resulting bioluminescence emission maximum is shifted to a longer wavelength up to 40 nm, which allows the use of some "yellow" Renilla luciferase mutants for in vivo imaging. However, the utility of coelenterazine-v in small-animal imaging has been hampered by its...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
