Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Negative net charge of EF-hand loop I can affect both calcium sensitivity and substrate binding pattern in mnemiopsin 2.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology - 13 Jun 2018

Vafa Masoomeh, Khalifeh Khosrow, Jafarian Vahab

Abstract excerpt

Mnemiopsin 2 from Mnemiopsis leidy has three Ca2+-binding motifs and has luminescence properties in the presence of calcium and coelenterazine. It has been reported that the pattern of calcium binding among EF-hand loops of various photoproteins is different. Here, we designed and constructed two variants of mnemiopsin 2 (E50G/D47N and E50G/E53T mutants) with modified EF-hand I in which the negative charge in the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Binding Sites
Share to Calcium

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Computational Biology

Share to Luminescence

Share to Luminescent Proteins

Share to Protein Binding

Share to Protein Engineering

Share to Spectrometry, Fluorescence

Source

Identifiers

PMID: 29770830
DOI: 10.1039/c8pp00058a

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Negative net charge of EF-hand loop I can affect both calcium sensitivity and substrate binding pattern in mnemiopsin 2.PMID 29770830Read ↗

Hyperactive Arg39Lys mutated mnemiopsin: implication of positively charged residue in chromophore binding cavity.Semantic proximity Read ↗

The Charge-driven Engineering of Mnemiopsin 2: Impact of Serine 35 Mutation on Activity and Stability.Semantic proximity Read ↗

Determination of structural elements on the folding reaction of mnemiopsin by spectroscopic techniques.Semantic proximity Read ↗

Expression and characterization of EF-hand I loop mutants of aequorin replaced with other loop sequences of Ca2+-binding proteins: an approach to studying the EF-hand motif of proteins.Semantic proximity Read ↗

Combined Mutational and Spectroscopic Study on the Calcium-Related Kinetic Effects on the VirChR1 Photocycle.Semantic proximity Read ↗

Role of four conserved aspartic acid residues of EF-loops in the metal ion binding and in the self-assembly of ciliate Euplotes octocarinatus centrin.Semantic proximity Read ↗

Metal-binding studies for a de novo designed calcium-binding protein.Semantic proximity Read ↗

Structural basis for unique color tuning mechanism in heliorhodopsin.Semantic proximity Read ↗

Hydrogen-bond networks between the C-terminus and Arg from the first α-helix stabilize photoprotein molecules.Semantic proximity Read ↗

Aequorin variants with improved bioluminescence properties.Semantic proximity Read ↗

Coelenterazine-v ligated to Ca2+-triggered coelenterazine-binding protein is a stable and efficient substrate of the red-shifted mutant of Renilla muelleri luciferase.Semantic proximity Read ↗

Chimeras of channelrhodopsin-1 and -2 from Chlamydomonas reinhardtii exhibit distinctive light-induced structural changes from channelrhodopsin-2.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.