Article
Crystal structure of nanoKAZ: The mutated 19 kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction with coelenterazine.
Biochemical and biophysical research communications - 29 Jan 2016
Tomabechi Yuri, Hosoya Takamitsu, Ehara Haruhiko, Sekine Shun-Ichi, Shirouzu Mikako, Inouye Satoshi
Abstract excerpt
The 19 kDa protein (KAZ) of Oplophorus luciferase is a catalytic component, that oxidizes coelenterazine (a luciferin) with molecular oxygen to emit light. The crystal structure of the mutated 19 kDa protein (nanoKAZ) was determined at 1.71 Å resolution. The structure consists of 11 antiparallel β-strands forming a β-barrel that is capped by 4 short α-helices. The structure of nanoKAZ is similar to those of fatty...
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