Article
The construction and application of diploid sake yeast with a homozygous mutation in the FAS2 gene.
Journal of bioscience and bioengineering - 1 Dec 2010
Kotaka Atsushi, Sahara Hiroshi, Hata Yoji
Abstract excerpt
In Japanese sake brewing, cerulenin-resistant sake yeasts produce elevated levels of ethyl caproate, an important flavor component. The FAS2 mutation FAS2-1250S of Saccharomyces cerevisiae generates a cerulenin-resistant phenotype. This mutation is dominant, and, in general, cerulenin-resistant diploid sake yeast strains carry this mutation heterozygously. Here we constructed diploid sake yeast with a homozygous...
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