Article
Combinatorial gene overexpression and recessive mutant gene introduction in sake yeast.
Bioscience, biotechnology, and biochemistry - 23 Mar 2009
Ano Akihiko, Suehiro Daisuke, Cha-Aim Kamonchai, Aritomi Kazuo, Phonimdaeng Prasart, Nontaso Ngarmnit, Hoshida Hisashi, Mizunuma Masaki, Miyakawa Tokichi, Akada Rinji
Abstract excerpt
Industrial yeast strains are generally diploid and are often defective in sporulation. Such strains are hence thought to be less tractable for manipulation by genetic engineering. To facilitate more reliable genetic manipulation of the diploid yeast Japanese sake, we constructed variants of this strain that were homozygous for a URA3 deletion, homozygous for either MATa or MATalpha, and homozygous for either the...
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