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Article

Isolation of auxotrophic mutants of diploid industrial yeast strains after UV mutagenesis.

Applied and environmental microbiology - 1 Jan 2005

Hashimoto Shinji, Ogura Mayumi, Aritomi Kazuo, Hoshida Hisashi, Nishizawa Yoshinori, Akada Rinji

Abstract excerpt

Auxotrophic mutants of the yeast Saccharomyces cerevisiae are usually isolated in haploid strains because the isolation of recessive mutations in diploids is thought to be difficult due to the presence of two sets of genes. We show here that auxotrophic mutants of diploid industrial sake yeast strains were routinely obtained by a standard mutant selection procedure following UV mutagenesis. We isolated His(-),...

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Identifiers

PMID: 15640203
DOI: 10.1128/AEM.71.1.312-319.2005
PMCID: PMC544216

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ATF1

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