Article
The Eurotransplant HLA-DRB oligonucleotide typing set.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Jan 2000
Giphart M J, Verduijn W
Abstract excerpt
Several sequence-specific oligonucleotides (SSO) were designed based upon available sequences of HLA-DRB1 genes which are associated with HLA-DR serotypes. Targets for these SSO were polymerase chain reaction (PCR) products using primers specific for the second exon of DRB genes. In view of practically, a hybridization system was developed which was independent of temperature or composition of the hybridization...
Topics
- Alleles
- Base Sequence
- Exons
- HLA-DR Antigens
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
