Article
[Gene amplification with PCR and sequence-specific HLA oligotyping].
Infusionstherapie und Transfusionsmedizin - 1 Jun 1992
Holzberger G, Frank R, Boehm B O, Seidl S
Abstract excerpt
Genetic polymorphism in the HLA-class II region has been identified by the analysis of the polymerase-chain reaction (PCR) products using sequence-specific oligonucleotide (SSO). The PCR-SSO method permits precise and direct analysis of allelic variations with as little as 1 microgram of genomic...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA Probes
- Gene Amplification
- HLA-DR Antigens
- HLA-DRB1 Chains
- Histocompatibility Antigens Class II
- Humans
- Microcomputers
- Molecular Sequence Data
- Oligonucleotide Probes
- Phenotype
- Polymerase Chain Reaction
- Signal Processing, Computer-Assisted
