Article
[Gene amplification with PCR and sequence-specific HLA oligotyping].
Infusionstherapie und Transfusionsmedizin - 1 Jun 1992
Holzberger G, Frank R, Boehm B O, Seidl S
Abstract excerpt
Genetic polymorphism in the HLA-class II region has been identified by the analysis of the polymerase-chain reaction (PCR) products using sequence-specific oligonucleotide (SSO). The PCR-SSO method permits precise and direct analysis of allelic variations with as little as 1 microgram of genomic DNA. The standardized, uniform hybridization and critical wash protocol of the Eurotransplant-typing kit [2] enables...
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