Article
Discrimination between HLA-DR1 (DRB1*0101) and DR'Br' (DRB1*0103) using sequence-specific oligonucleotide probes.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Jan 2000
Bidwell J L, Bidwell E A
Abstract excerpt
Serological identification of the HLA-DQw1(w5)-associated or HLA-DQw3(w7)-associated DR'Br' (DRB1*0103) allele cannot be accomplished in the presence of a second DQw1(w5)-positive or DQw3(w7)-positive haplotype, respectively. DNA-restriction fragment length polymorphism (RFLP) analysis assists in identification of DR'Br', though not in the presence of DR1. We describe an alternative or complementary method for...
Topics
- Alleles
- Base Sequence
- DNA Probes
- HLA-DR1 Antigen
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
