Article
Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication.
The Journal of clinical investigation - 1 Sept 2010
Stanton Richard J, Baluchova Katarina, Dargan Derrick J, Cunningham Charles, Sheehy Orla, Seirafian Sepehr, McSharry Brian P, Neale M Lynne, Davies James A, Tomasec Peter, Davison Andrew J, Wilkinson Gavin W G
Abstract excerpt
Human cytomegalovirus (HCMV) in clinical material cannot replicate efficiently in vitro until it has adapted by mutation. Consequently, wild-type HCMV differ fundamentally from the passaged strains used for research. To generate a genetically intact source of HCMV, we cloned strain Merlin into a self-excising BAC. The Merlin BAC clone had mutations in the RL13 gene and UL128 locus that were acquired during...
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