Article
Repair of an Attenuated Low-Passage Murine Cytomegalovirus Bacterial Artificial Chromosome Identifies a Novel Spliced Gene Essential for Salivary Gland Tropism.
Journal of virology - 27 Oct 2020
Redwood Alec James, Masters Laura Lee, Chan Baca, Leary Shay, Forbes Cathy, Jonjić Stipan, Juranić Lisnić Vanda, Lisnić Berislav, Smith Lee Martin
Abstract excerpt
The cloning of herpesviruses as bacterial artificial chromosomes (BACs) has revolutionized the study of herpesvirus biology, allowing rapid and precise manipulation of viral genomes. Several clinical strains of human cytomegalovirus (HCMV) have been cloned as BACs; however, no low-passage strains of murine CMV (MCMV), which provide a model mimicking these isolates, have been cloned. Here, the low-passage G4...
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