Connecting mutations of the RNA polymerase II C-terminal domain to complex phenotypic changes using combined gene expression and network analyses.

Abstract excerpt

The C-terminal domain (CTD) of the largest subunit in DNA-dependent RNA polymerase II (RNAP II) is essential for mRNA synthesis and processing, through coordination of an astounding array of protein-protein interactions. Not surprisingly, CTD mutations can have complex, pleiotropic impacts on phenotype. For example, insertions of five alanine residues between CTD diheptads in yeast, which alter the CTD's overall...

Topics

• Amino Acid Sequence
• Cell Separation
• Chromosomes, Fungal
• DNA, Fungal
• Flow Cytometry
• Gene Expression
• Molecular Sequence Data
• Mutation
• Oligonucleotide Array Sequence Analysis
• RNA Polymerase II
• Saccharomyces cerevisiae