Article
Response of dynamic structure to removal of a disulfide bond: normal mode refinement of C77A/C95A mutant of human lysozyme.
Protein science : a publication of the Protein Society - 1 Jan 1994
Kidera A, Inaka K, Matsushima M, Go N
Abstract excerpt
In order to investigate the response of dynamic structure to removal of a disulfide bond, the dynamic structure of human lysozyme has been compared to its C77A/C95A mutant. The dynamic structures of the wild type and mutant are determined by normal mode refinement of 1.5-A-resolution X-ray data. The C77A/C95A mutant shows an increase in apparent fluctuations at most residues. However, most of the change...
Topics
- Binding Sites
- Crystallization
- Crystallography, X-Ray
- Disulfides
- Humans
- Models, Molecular
- Molecular Structure
- Muramidase
- Mutation
- Structure-Activity Relationship
- Thermodynamics