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Article

Remarkable improvement in the heat stability of CutA1 from Escherichia coli by rational protein design.

Journal of biochemistry - 1 Oct 2010

Matsuura Yoshinori, Ota Motonori, Tanaka Tomoyuki, Takehira Michiyo, Ogasahara Kyoko, Bagautdinov Bagautdin, Kunishima Naoki, Yutani Katsuhide

Abstract excerpt

To enhance the heat stability of the CutA1 protein from Escherichia coli (EcCutA1) so that it has comparable stability to CutA1 from Pyrococcus horikoshii with a denaturation temperature (T(d)) of 150°C, we used the Stability Profile of Mutant Protein (SPMP) to examine the structure-sequence (3D-1D) compatibility between the conformation of EcCutA1 and its native sequence [J. Mol. Biol., 248, 733-738, (1995)]. We...

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PMID: 20639520
DOI: 10.1093/jb/mvq079

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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