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Article

Thermostabilization of Bacillus subtilis GH11 xylanase by surface charge engineering.

International journal of biological macromolecules - 1 Jun 2016

Alponti Juliana Sanchez, Fonseca Maldonado Raquel, Ward Richard J

Abstract excerpt

Aiming to improve thermostability of the mesophilic xylanase A from Bacillus subtilis (XynA), five single mutants (S22E, S27E, N32D, N54E and N181R) were used to construct a random combinatorial library, and screening of this library for thermostable XynA variants identified a double mutant (S22E/N32D). All 6 mutants were expressed in Escherichia coli (BL21) and purified. Xylanase activity showed all mutants have...

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Identifiers

PMID: 26955749
DOI: 10.1016/j.ijbiomac.2016.03.003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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